Does the large size of IgM limit the effectiveness of certain fluorophore- or enzyme-conjugated secondary antibodies in
Recently I was staining a section and noticed my IgM signals looked patchy. It made me wonder if the bulky size of IgM can limit how well fluorophore-tagged secondaries bind?
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From what I’ve seen, that size question comes up a lot when people switch between antibody classes. I ran into something similar during a messy optimization phase and went down a rabbit hole reading about structure and behavior. One page that helped me frame it differently was this note on the lgm full form , mostly because it reminded me how different IgM really is in physical terms. I don’t think it’s automatically a problem, but it definitely changed how I interpret uneven staining instead of blaming my hands right away.